Step 1: Primary Tests
Enter the results for your unknown organism. These first five tests will narrow down the possibilities.
Step 2: Secondary Tests
Now, let's refine the results. Please enter the data for the remaining tests. Select "N/A" if you did not perform the test.
Step 3: Identification Result
Your organism has been identified. BactID uses a small dataset of soil bacteria to suggest identity. Your organism may be phenotypically similar but have a different identity.
Complete Biochemical Profile:
No organism in the database matches all of your selected criteria.
This could be due to a contaminated plate, a misread "Variable" test, or the organism is not in this database. BactID uses a small dataset of soil bacteria to suggest identity. Please double-check your tests.
Your secondary tests point to ****.
However, this organism's known profile **conflicts** with the primary tests (Gram, Catalase, etc.) you entered on Step 1. This indicates a high probability of a misread test based on the bacteria in this database.
Step 4: 16S Validation
Please perform 16S rDNA sequencing on your isolate. Analyze the results to determine the genus, then enter your findings in the form below.
References & Resources
Biochemical Characterization
- Bergey's Manual of Systematics of Archaea and Bacteria - The definitive guide to bacterial classification.
- MicrobeWiki - A student-edited microbiology resource.
- BacDive - Database for standardized bacterial information.
- Biochemical Test Methods - ASM Protocol Collection: standardized methods for microbiological tests.
- Tiny Earth - Tiny Earth is a course-based undergraduate research experience (CURE), an international network, and an antibiotic discovery pipeline.
BactID
- Source Code - Github respository for BactID files.
- BactID Gemini Prompts - A brief summary of the steps use to create the BactID tool.
- Add a Bacterium - Fill out this form to add a bacerium to the BactID databse.